Fig. 9. Mitochondrial potential can be maintained by the reversal of the ATP synthase. (A-C) Simultaneous measurement of [Ca²⁺]_c (using fura 2 AM) and of m (using Rhod123) in mature eggs. Where indicated, oligomycin (60 µM), FCCP (1 µM), malonate (20 mM) and rotenone (5 µM) are added to the bath. (D,E) Schematic representations of a mitochondria and the effect of mitochondrial inhibitors on the proton fluxes generated at the respiratory chain (D, CN^–, rotenone, malonate) or at the F₀/F₁ synthase (E, oligomycin).