Fig. 4. Knocking-down iro7 results in an anterior expansion of vhnf1 expression. (A,B,D,E) Whole-mount in situ hybridisation with the probes indicated (bottom left, colour coded) on Moz7 injected (A,D) or control (B,E) embryos. All pictures are dorsal views of whole-mount (A,B) or flat-mounted (D,E) embryos, with anterior towards the top. (C) A mean increase of 34% (t-test; P<0.001) of the AP length of vhnf1 expression domain in Moz7-injected embryos compared with control embryos.