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Fig. 6. vhnf1 represses iro7 expression. (A-D) Double in situ hybridisation with probes for iro7 (blue) and krx20 (red) on vhnf1 homozygous (A,C) or control (B,D) embryos. (E) The statistical analysis of the posterior expansion of the iro7 expression domain in vhnf1 homozygous mutant embryos at 1-2 s. (F-H) In situ hybridisation with an iro7 probe on embryos injected with vhnf1 (20ng/µl) and lacZ (F,G) or with vhnf1Q139E (25 ng/µl) (H) RNAs. (G) Immunohistochemistry with an antibody directed against ß-galactosidase.