Fig. 4. Immunohistochemical and behavioral characterization of daughter cells generated from NS-dividing cells. (A) A ^BPP_NS-div cell observed in an E13 slice adopted a unipolar shape and subsequently divided to produce two Hu⁺ cells, one of which inherited the pia-connected process. Panels A' and A' show merged views of confocal images taken using a 40x (A') or 100x (A'') objective lens. (B) After undergoing bipolar-to-unipolar change, a ^BPP_NS-div cell captured in an E13 slice generated two Hu⁺ cells (B', 100x), one of which (cell ‘a’) ascended more quickly than its sister (cell ‘b’), having entered the immature cortical plate (CP) by 20.4 hours. (C) A P_NS-div cell observed in an E14 slice. Division may have occurred by 5.0 hours. Both daughter cells initially had two or three non-radially oriented processes in the subventricular zone (SVZ) (10.0-21.0 hours) and later became more radially oriented and Hu⁺ [C', 40x; C'', photographed at three different planes (100x)] in the lower intermediate zone (28.6 hours), resembling locomotion neurons (Nadarajah et al., 2001). Scale bars: 10 µm in A',A',B',C',C'.