Fig. 5. NS-dividing cells in E13-E14 cerebral walls are mostly Hu⁺. (A-A'') Hu and pH3 double staining allows for the identification of cells undergoing division in E13 cerebral wall section. Two NS mitoses were clearly Hu⁺ (A'' shows magnified images of arrowed cell, two different confocal planes at 100x). S-dividing cells (A') were completely negative for Hu. (B) Quantification of the stage-dependent changes in the proportion of the pH3⁺ NS-dividing cells that were Hu⁺ demonstrates decreased anti-Hu reactivity with development (n=332 cells at E13, 267 cells at E14, 221 cells at E17, and 246 cells at P0). Scale bar: 10 µm.