Fig. 6. Effects of hen3 mutations on leaf size and leaf epidermal cell size. (A-C) Dissected rosette leaves 1-7 (arranged from left to right) from hen3 (top) and wild-type (bottom) plants that were grown side by side. (A) hen3-1 and wild type. (B) hen3-2 and wild type. (C) hen3-3 and wild type. Note that the first two leaves are not affected as much as the later leaves by the hen3 mutations. The hen3 mutations all lead to a reduction in leaf size. (D) Area (cm²) of the fully expanded fifth leaf from wild-type and hen3 genotypes. Ten leaves were measured for each genotype. (E) Surface area (µm²) of adaxial epidermal cells on the fifth leaf of wild-type and hen3 genotypes. The number of cells measured was 95, 141, 173 and 143 for wild type, hen3-1, hen3-2 and hen3-3, respectively. The large standard error is largely due to the intrinsic variation in cell size on the leaf epidermis. (F) Number of epidermal pavement cells in a 70,000 µm² area from similar positions in the fifth leaves of various genotypes. Measurements were performed on SEM images of three leaves from each genotype. The error bars in D-F represent standard errors.