Fig. 7. Gata1 levels decrease during terminal erythroid differentiation. (A) Whole cell extracts from wild-type foetal liver cells, after day 0, 1 and 2 of hanging drop culture were analysed by western blotting using antibodies against Gata1 with nucleophosmin as loading control. Two samples at day 0 are shown and cultures were done in triplicate. (B) Jo2 treatment of wild-type cells. Whole cell extracts at day 2 after standard (St) culture and culture with Jo2 1 (20 µg/ml) or Jo2 2 (40 µg/ml) were analysed by western blotting using antibodies against Gata1, and nucleophosmin as a loading control. (C) Jo2 treatment of erythroid cells from Gata1-overexpressing foetuses. Whole cell extracts at day 0 and 2 after standard culture and culture with Jo2 1 (20 µg/ml) were analysed by western blotting using antibodies against Gata1 with nucleophosmin as a loading control. Extracts from wild-type, X^OXX female and X^OXY male foetal liver cells are shown.