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Fig. 6. The sub-cellular distribution of Wg protein in the Wg-producing cells is altered in ds mutant cells. (A,B) Third instar discs containing ds38k clones generated by the Minute technique. Clones were induced during second instar and are marked by the absence of armZ (red). (A) In ds38k cells, the gradient of extracellular Wg (green) is expanded with respect to the adjacent wild-type cells (arrowheads). (B) A cross-section from the region marked by the white line in A, showing a higher accumulation of Wg protein at the apical surface of ds mutant cells (compare with wild-type cells, red marker). Wg is uniformly distributed along the apical-basal axis. Note that the mislocalization of Wg also affects the wild-type cells adjacent to ds38k mutant cells (bracket). Wg expression is shown in the green channel.