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Fig. 5. Analysis of cell proliferation in Osr2tm1Jian/tm1Jian mutants at E13.5. (A-C) Frontal sections showing BrdU-labeled cell nuclei (blue color) in wild type (A), heterozygous (B) and homozygous mutant (C) palatal shelves at E13.5. Arrows indicate medial side of the palatal shelves. (D) Comparison of the percentage of BrdU-labeled cells in a fixed area of palatal mesenchyme in wild-type (+/+), heterozygous (+/-) and homozygous mutant (-/-) embryos. Standard deviation values were used for the error bars.