Fig. 2. gld-1 mediates the translational repression activity of the GRE. The distal gonad arms are outlined and the autofluorescent intestine is visible in A and C. (A) GH::pie-1 3' UTR is expressed in nuclei throughout the proximal and distal germline. (B) GH::2X GRE is expressed in proximal germline nuclei, but not distal germline nuclei. (C) GH::2X GRE is ectopically expressed in distal germline nuclei following gld-1 RNAi. The characteristic gld-1(–) proximal germline tumor is evident. (D) Multi-second exposure of a dissected GH::2X GRE gonad arm. Weak expression is observed in the distal germline cytoplasm. (E) RNase protection assays with pal-1 and gfp probes and 60 µg of total RNA from wild-type or gld-1 (RNAi) GH::2X GRE L4 larvae (integrated transgene). gld-1 does not destabilize pal-1 or GH::2X GRE mRNA.