Fig. 3. gld-1 represses the distal germline expression of both PAL-1 and MEX-3. The distal gonad arms are outlined. (A,B,D,E) Anti-PAL-1 (A,B) and anti-MEX-3 (D,E) staining in wild-type (A,D) and gld-1(RNAi) (B,E) gonad arms. (A,D) No expression is detected in the distal germline. PAL-1 is detectable in immature oocytes in 53% of gonad arms (n=49) and the staining shown here is an extreme example illustrating the maximal PAL-1 expression observed. (B,E) Ectopic expression is observed in the distal germline. (C) Anti-PAL-1 staining in a gld-1 (RNAi); mex-3 (RNAi) gonad arm. Compared to gld-1 RNAi worms, significantly more ectopic PAL-1 is observed in the distal germline (see text for quantitation).