Fig. 2. Rescue of `canonical' Wg pathway features following overexpression of Wg, Arm^act or Dsh in wg^– embryos. Analysis of the different cuticle phenotypes from the following crosses: females wg^CX4/CyoftzZ;daGal4 x males wg^CX4,UASWg/CyoftzZ (wg>da>Wg), wg^CX4,UASArm^act/CyoftzZ (wg>da>Arm^act) and wg^CX4/CyoftzZ;;UASDsh (wg>da>Dsh). The embryos shown above are stained for Engrailed. wg>da>Wg: 44.5% of the embryos overexpress Wg and present a fully naked cuticle, and are organised in two categories of different strength (30%, 14.5%). 21% of the embryos overexpress Wg and have a wild-type phenotype (out of the 54% showing a wild-type phenotype, 33% do not overexpress Wg). Wg overexpression in wg^– embryos is thus able to rescue the naked cuticle identity and to some extent suppress the denticules specification. En expression is rescued to normal. wg>da> Arm^act: 39.4% of the embryos overexpress Arm^act and show a fully naked cuticle, and 30.6% of the embryos overexpress Arm^act and show a wild-type phenotype. Arm^act overexpression is thus able to rescue the naked cuticle identity. En expression, as shown by the above embryo, is normal. wg>da>Dsh: all the embryos from this cross overexpress Dsh. A mild rescue of the naked cuticle identity (see Fig. 5C') is observed in 21% of the embryos (33% are wg^–). A partial rescue of En expression is observed.