Fig. 5. Expression of mesenchymal, epithelial and stromal markers in the embryonic kidney expressing human SPRY2. Gdnf, which is expressed in the nephrogenic mesenchyme, is downregulated by human SPRY2 (compare A with A' and A''), while its Ret receptor encoding gene remains normally expressed irrespective of the presence of human SPRY2 (B-B''). Wnt11 expression is reduced in response to human SPRY2 expression (compare C with C',C''). Wnt11 also appears in the stromal cells of the transgenic kidney (C',C''). Pax2 and Bmp4 gene expression is unchanged (D-E''), while the stromal marker Foxd1 is reduced (F-F'') because of ectopic human SPRY2 (A-F''; E.15.5). Scale bar: 100 µm.