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Fig. 2. Morpholinos RPTPmo1 and RPTPmo2 specifically block RPTP{psi} translation. (A) Morpholino target sequences. Part of the 5' UTR of the RPTP{psi} mRNA is shown illustrating the binding sites of the two morpholinos RPTPmo1 (spanning the ATG) and RPTPmo2 (binding 5' to ATG). (B) In vitro assay showing specific inhibition of RPTP{psi} translation in the presence of RPTPmo1 and RPTPmo2 morpholinos. RPTP{psi} cDNA (0.5 µg) was transcribed and translated in vitro in the presence of 35S-labeled methionine in the absence of morpholino (lane 2) or in the presence of 5-base mismatch control morpholino (5m, lane 3), unrelated morpholinos, e.g. against deltaC (DlC, lane 4) and various concentrations of RPTP{psi} morpholinos (RPTPmo1, lanes 5 and 6; RPTPmo2, lanes 7 and 8). Lane 1, in vitro transcription translation in the absence of RPTP{psi} cDNA. 35S-RPTP{psi} (arrow) was analysed by SDS-PAGE followed by autoradiography.