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Fig. 3. Grem1 is required for propagation of Shh in the limb bud mesenchyme and for FGF gene expression in the AER. (A) Shh expression in wild-type and Grem1 (Gre{Delta}ORF/{Delta}ORF)-deficient limb buds during E10.0 and E11.0. The Shh expression domain is activated but not propagated in mutant limb buds. Posterior is towards the bottom and distal towards the right. (B) Expression of Fgf8 is activated normally (E9.5), but the domain remains broader and patchy in mutant limb buds (arrowheads, E9.75). (C) Neither Fgf4 nor Fgf9 expression is activated in mutant limb buds (E10.25). All limb buds shown are fore limb buds. In B,C, posterior is towards the bottom and dorsal towards the left.