Fig. 6. Unc-GFP localization in spermatogenesis. Confocal images of spermatids (A-C) and spermatocytes or gonial cells (D-G) expressing UNC-GFP (green). Anti-ß-tubulin (blue) labels axonemal and cytoplasmic microtubules in all panels, and propidium iodide (red) stains DNA in B-D. (A) Low-magnification image of the of the testis tip, showing UNC-GFP in elongated spermatids at the apical end of the flagella. (B) Round spermatids immediately following meiosis II show a single GFP-labeled centriole associated with each nucleus. (C) UNC-GFP is associated with the centriole adjunct (arrowhead) in elongating spermatids, but is not present in mature spermatids with fully condensed nuclei (open arrowhead). (D) Primary spermatocytes in meiosis I, showing paired centrioles at each pole of the meiotic spindle. (E,F) Co-expression of UNC-GFP and centrosomin (red) in gonial cells (arrow). (G) Colocalization of UNC-GFP and centrosomin (red) in dividing spermatocytes. Scale bars: 10 µm in A; 5 µm in B-G.