Fig. 6. (A-D) RC2 immunostaining on coronal sections of mouse telencephalic vesicles at E13 and P0. (A,C) Heterozygous animals; (B,D) Tenascin C (TNC)-deficient animals. A reduction in the intensity of RC2 staining is observed in the brains of TNC null animals at both ages, but note the normal morphology of the processes at P0. (E) The percentage of RC2-positive cells in dissociated cell populations from heterozygous and homozygous animals during different stages of CNS development. Note the decrease in the proportion of RC2+ cells in the homozygous animals at later stages, showing a reduction in the number of radial glia. ^*P<0.05. c, cortex; s, striatum; lv, lateral ventricle.