Fig. 6. Cells expressing high levels of ß1 generate more neurospheres. Dissociated (A) and intact (B) neurospheres stained for ß1 (red in A, arrow in B). Note the presence of a cell population expressing high levels of ß1. Nuclei in A were counterstained with DAPI (blue). (C) Neurospheres grown in EGF were dissociated, labelled with a monoclonal anti-ß1 antibody conjugated with FITC and sorted into two groups – high expressers (box 1) and moderate expressers (box 2) – as discussed in the text. (D) Neurospheres grown in FGF2 were dissociated, labelled with a monoclonal anti-ß1 antibody conjugated with FITC and sorted into two groups – high expressers (box 3) and moderate expressers (box 4) as above. (E) Graph showing the number of secondary neurospheres formed from the sorted cell populations after one week in culture. The differences between both 1 and 2 (high and moderate ß1 expressers in EGF), and between 3 and 4 (high and moderate ß1 expressers in FGF2), are statistically significant (P<0.001). Scale bar: 20 µm for A,B.