Fig. 7. Impaired cyclin D1 and Bcl-X_L expression in MTA1-TG mice. (A) Western blot analysis of cyclin D1 and cytokeratin 18 in wild-type and MTA1-transgenic from virgin 12 weeks, pregnant 15 days and lactation day 2 mammary glands. (B) Northern blot analysis of cyclin D1 mRNA level in MTA1-MCF7 overexpressing cell line. mRNA was extracted from MCF-7/PCDNA and MCF-7/MTA1 cells. Northern blots were prepared and probed with cDNAs encoding cyclin D1 and K18 as a control to permit normalization for epithelial content of the mRNA samples. (C) Western blot of total proteins from the inguinal mammary glands at various stages of development, virgin 12 weeks, pregnancy day 10, and lactation day 2 with anti-Bcl-X_L and anti-vinculin antibody on the same blot. (D) Western blot analysis of Bcl-X_L and vinculin in HC11/pcDNA and HC11/MTA1-overexpressing cells.