Fig. 3. Cyclin B1 is not degraded during meiosis in cul-2(RNAi) embryos. (A) DIC (top) and epifluorescence (bottom) images of wild-type, cul-2(RNAi) and zyg-11(RNAi) gravid adults expressing a cyb-1::GFP transgene. Embryo stages are labeled. Note that CYB-1::GFP signal is absent in the wild-type pronuclei stage embryo (immediately after meiosis) but perdures in cul-2(RNAi) and zyg-11(RNAi) embryos. Intestine autofluorescence is observed above the oocyte and eggs. Scale bar: 10 µm. (B) Graph of CYB-1::GFP epifluorescence intensity at times post-fertilization. Data was collected under the same conditions for each embryo and is presented in equivalent arbitrary units. The completion of meiosis is denoted by a thick striped bar. (C) Meiosis II timing for individual embryos of the strains listed, derived from histone H2B::GFP movies.