Fig. 5. Inverted AP polarity during meiosis II delay in the absence of zyg-11 or cul-2 function. Wild-type (A-D), zyg-11(RNAi) (E-H), cul-2(RNAi) (I-L), and cyb-3(RNAi) (M-P) embryos during meiosis II, and wild-type embryos during the first mitotic cell cycle (Q-T), stained with antibodies against -tubulin (green in merged image) and a polarity marker as indicated (PAR-1, PAR-2, PAR-3 or PAR-1+PGL-1 to detect P granules; red in merged image and viewed alone in the top panel); DNA is shown in blue in merged image. Scale bar: 10 µm. Note that enrichment of PAR proteins and P granules in the absence of zyg-11 or cul-2 function is somewhat variable during meiosis II, and is less pronounced, both in intensity and in lateral extent, than in wild-type embryos during the first mitotic cell cycle.