(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. zyg-11 can prevent polarity establishment independently of promoting meiosis II cell cycle progression. (A-D) 25°C mat-1(ax161) or 25°C mat-1(ax161) zyg-11(RNAi) embryos stained with antibodies against {alpha}-tubulin (green) and PGL-1 to detect P granules (red); DNA is shown in blue. Depicted are embryos from the metaphase I (A,C) and the metaphase I (late) (B,D) categories (see Fig. 4). Insets show magnified views of meiotic chromosomes. Scale bar: 10 µm; width of inset represents ~7 µm. (E,F) Live ncc-1(RNAi) [heterozygous for zyg-11(mn40)] or ncc-1(RNAi) zyg-11(mn40) embryos during meiosis expressing GFP-PAR-2 (green) and stained with Hoechst 33342 to view DNA (blue in merged image). Insets show magnified views of meiotic chromosomes; width of inset represents ~8 µm. Left panels show a DIC image of the same embryo. (G-J) Live mat-1(RNAi) [heterozygous for zyg-11(mn40)] or mat-1(RNAi) zyg-11(mn40) embryos expressing GFP-PAR-2 (green) and stained with Hoechst 33342 to view DNA (blue in merged image). Insets show magnified views of meiotic chromosomes; width of inset represents ~8 µm. Scale bar: 10 µm.