Fig. 6. Monocular retinal blockade of ERK activation. (A,B,D,E) Representative images of both sides of the dLGN of rats that received monocular injections of beads-U0126 (A,B; n=7) or vehicle (D,E; n=7). Projections from the treated eye are labeled with CTB-Alexa Fluor 488 (green) and those from untreated eye with CTB-Alexa Fluor 594 (red). Note that in the U0126-treated animals both contralateral (A) and ipsilateral (B) fibers from the treated eye (green) occupy smaller territories then the corresponding projections from the untreated eye (red, A,B). Conversely, in control rats (D,E), fibers from both eyes occupy similar areas in the dLGN. (C,F) Cresyl violet staining of cells in the GCL. Living cell density in the retinas of rats monocularly injected with U0126 (C; 69.3±6.1 cells per field, n=5) is not statistically different (Student's t-test, P>0.05) from that of vehicle-treated retinas (F; 66.1±2.5 cells per field, n=4). Scale bars: 200 µm for A,B,D,E; 20 µm for C,F.