Fig. 8. FGF9 does not induce Sertoli markers but does induce Scc. Nuclear FGFR2 (green) was specific to Sertoli cells within the cords of wild-type XY gonads at 12.5 dpc (A). In Fgf9^-/- XY gonads, FGFR2 was found in somatic cells throughout the gonad, but was not found in the nuclei of any cells (C), in a similar pattern to the Fgf9^+/- or Fgf9^-/- XX gonad (B,D). (E-G) FGFR2 is nuclear in cultured XY gonads (E), but not in XX gonads (F). FGF9 did not induce the nuclear localization of FGFR2 in cultured XX gonads (G). Germ cells and vasculature are red. (H) Using in situ hybridization, Scc labels Leydig cells in the interstitium of XY gonads and Sox9, Amh (Mis in figure) and Dhh label Sertoli cells within testis cords. These genes were not expressed in XX gonads at this stage. FGF9 did not induce markers associated with Sertoli cell differentiation, but did induce Scc expression in XX gonads. Scale bars: 50 µm in A-G; 250 µm in H.