Fig. 4. Abnormal expression of Xbap in FoxF1 knockdown embryos.
(A,B) Whole-mount in-situ hybridization showing Xbap expression on
both sides of a CoMo-injected stage 35/36 embryo. A line with a letter in (A)
indicates the position of the section in (I). (C,D) Whole-mount in-situ
hybridization of Xbap RNA to a FoxF1Mo-injected stage 35/36
embryo. Xbap expression is present on the uninjected side (C) but is
absent on the injected side (D). A line with a letter in (C) indicates the
position of the section in (J). (E,F) Whole-mount in-situ hybridization
showing FoxF1 expression on both sides of a CoMo-injected stage 35/36
embryo. A line with a letter in (E) indicates the position of the section in
(K). (G,H) Whole-mount in-situ hybridization of FoxF1 RNA to a
FoxF1Mo-injected stage 35/36 embryo. FoxF1 expression is
present on both sides of the embryo. A line with a letter in (G) indicates the
position of the section in (L). (I) A section through the embryo in (A) shows
that Xbap is expressed in the CoMo-injected (shown as right) side as
well as on the uninjected (left). (J) A section through the embryo in (C)
shows that Xbap is expressed on the uninjected (left) side of a
FoxF1Mo-injected embryo but not on the injected (right) side of a
tadpole. (K) A section through an embryo hybridized with FoxF1 RNA
shows that the lateral plate mesoderm is present on the CoMo-injected (right)
side as well as on the uninjected side (left). (L) A section through an embryo
hybridized with FoxF1 RNA shows that the lateral plate mesoderm is
present on the FoxF1Mo-injected (right) side as well as on the
uninjected side (left). Arrows point to the anterior lateral plate
mesoderm.