Fig. 3. Vegetative phenotypes observed with the sty gram double mutant. For comparison the gram mutant is shown in A, F and at the left in D. (A-C) 5- to 6-week-old seedlings. Notice irregular phyllotaxis in B and fused cotyledons above a `bulge' wrapping the arrested shoot tip in C. (D) Comparison of leaves from the fourth node of 10-week-old plants. (E) Detail of a 10-week-old plant with needle-like (arrow) and filamentous leaves (^*). (F,G) SE micrographs of freeze-fractured needle-like leaves. The sub-epidermal cell layer is highlighted by false green colour. (H) Histological section of the central vein in a radialised sty gram leaf. p and x indicate phloem and xylem elements, respectively. The mutants in E-H are in the JI75 genetic background. Scale bars: 5 mm (A-E); 50 µm (F,G) and 25 µm (H).