Fig. 7. Aberrant migration and defective differentiation of the dorsal horn neurons in wild-type and Rnx^-/- and Drg11^-/- mutant embryos. (A) Neurons labeled with BrdU at E11.5 are not detected in the outer layer of laminae I-II (bracket) in wild-type embryo at E14.5 (inset). (B) Neurons labeled with BrdU at E11.5 are detected in the outer layer of laminae I-II in Rnx^-/- embryo (inset, bracket). (C) Quantitative comparison of the number of BrdU⁺ neurons in the medial one-third (Med 1/3) and lateral two-thirds (Lat 2/3) regions between wild-type and Rnx^-/- embryos (^*P<0.001). (D,E) Comparison of the distribution pattern of neurons labeled with BrdU at E11.5 in the dorsal horn of E14.5 wild-type (D) and Drg11 mutants (E). (F) Quantitative analysis of the numbers of BrdU-labeled neurons in the medial and lateral regions of wild-type and Drg11 mutant embryos (^*P<0.001). (G,H) MAP2 expression in the dorsal horn of wild-type (G, bracket) and Rnx mutant embryos (H, bracket). MAP2 expression of the dorsal horn in wild-type (I, bracket) and Drg11^-/- mutant embryos (J, bracket). Scale bars: in B and C, 100 µm for A and D, respectively; 50 µm in insets in B and E; 100 µm in G-J.