Fig. 8. Selective block of the ingrowth of cutaneous afferents in the dorsal spinal cord of Lmx1b^-/- mutant embryos at E14.5. (A,B) Immunocytochemical detection of TrkA in the dorsal horn of wild-type (A) and Lmx1b^-/- mutant (B) embryos. Arrows indicate the superficial laminae region. Brackets outline laminae I-II region. (C,D) Peripherin detection in the dorsal horn of wild-type (C) and Lmx1b mutant embryos (D) appears unchanged. Arrows indicate peripherin-labeled presumptive proprioceptive afferents. (E,F) DiI labeling of primary afferents in the dorsal horn of wild-type (E) and Lmx1b mutant embryos (F). Arrowheads indicate presumptive mechanoreceptor afferents and arrows indicate presumptive muscle proprioceptive afferents. Asterisks and brackets outline laminae I-II region. (G,H) Sema3c expression in wild-type (G) and Lmx1b mutant embryos (H). (I,J) Nrp1 expression in wild-type (I) and Lmx1b mutant embryos (J). (K,L) Plexin A2 expression in wild-type (K) and Lmx1b mutant embryos (L). (M,N) Slit1 expression in wild-type (M) and Lmx1b mutant embryos (N). (O,P) Robo2 expression in wild-type (O) and Lmx1b mutant embryos (P). (Q,R) Netrin 1 expression in wild-type (Q) and Lmx1b mutant embryos (R). Arrows in G-S indicate laminae I-II. Arrowheads in Q and R indicate the lateral region of laminae I-II. Scale bars: 200 µm in A, B; 100 µm in C-R.