Fig. 1. D204 is a candidate downstream gene of Islet3 and Islet2. (A,A') D204 mRNA expression in 24 hpf normal (A) and LIM^Isl-3-overexpressing (A') zebrafish embryos. The expression in tectum was reduced in LIM^Isl-3-overexpressing embryo (arrows in A and A'). The embryos overexpressing LIM^Isl-3 lack optic vesicles (ov) and MHB (brackets). (B,B',D,D') The peripheral axons of both the trigeminal sensory ganglion neurons (arrowheads in B and black circles in B') and Rohon-Beard neurons (black circles in D and D') were eliminated by overexpression of LIM^Isl-2 (B',D'), while their central axons (arrows in B and B') remained intact as in normal embryos at 24 hpf. The axons were stained by anti-acetylated -tubulin antibody. Embryos in B and D are normal controls. (C,C',E,E') D204 expression in the trigeminal sensory ganglion neurons (arrows) and Rohon-Beard neurons (arrowheads) were reduced in LIM^Isl-2-overexpressing 21 hpf embryos (C',E'), while the expression of D204 in the hindbrain remained intact (arrowheads in C'). Embryos in C and E are normal controls. (A,A',C,C') Dorsal views; anterior leftwards. (B,B',D,D',E,E') Lateral views; anterior leftwards; dorsal towards the top. Scale bar: 20 µm in A,C; 10 µm in B,D,E.