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Fig. 6. Transgenic overexpression of dnPlexinA4-GFP in the Rohon-Beard neurons caused reduction in the number of branching of the peripheral axons of Rohon-Beard neurons. Anti-acetylated {alpha}-tubulin antibody staining of 26 hpf embryos of the transgenic zebrafish (B), which expresses dnPlexinA4-GFP fusion protein in the Rohon-Beard neurons (black circles), showed significant reduction in the number of branching points of the peripheral axons compared with that in the normal 26 hpf embryo (A,C), especially in the D2 region (D). Lateral view; anterior is leftwards; dorsal is towards the top. Scale bar: 10 µm.