Fig. 3. Quantitative analysis of the effect of different treatments on the number of primary dendrites. Cortical slices were grown under control conditions (Ctr), or in medium supplemented with 200 ng/ml HGF (HGF), 1 µg/ml anti-HGF (aHGF), 200 ng/ml HGF plus 1 µg/ml anti-HGF pre-incubated for 1 hour before addition to the cultures (HGF + a1), 200 ng/ml HGF plus 3 µg/ml anti-HGF pre-incubated for 1 hour before addition to the cultures (HGF + a2), 200 ng/ml BDNF (BDNF) or HGF + BDNF. The bar charts illustrate the total number of primary dendrites. ^*P<0.05. Between 50 and 60 individual neurons were analysed under each condition.