Fig. 4. Quantitative analysis of the effect of different treatments on the apical and basal compartment of dendritic arbors. Cortical slices were grown under control conditions (Ctr), or in medium supplemented with 200 ng/ml HGF (HGF), 1 µg/ml anti-HGF (aHGF), 200 ng/ml HGF plus 1 µg/ml anti-HGF pre-incubated for 1 hour before addition to the cultures (HGF+a1), 200 ng/ml HGF plus 3 µg/ml anti-HGF pre-incubated for 1 hour before addition to the cultures (HGF+a2), 200 ng/ml BDNF (BDNF) or HGF+BDNF. The bar charts illustrate percent change on either dendrite length or number of branches relative to untreated controls. ^*P<0.05, ^**P<0.01. Between 50 and 60 individual neurons were analysed under each condition.