Fig. 6. Effects of HGF and anti-HGF treatment on the growth rate of the same layer 2 pyramidal neurons imaged at two time points in culture. Cultures were treated with either 200 ng/ml HGF or 1 µg/ml anti-HGF function-blocking antibodies. A number of sampled neurons per condition were each scanned at two different time points: 18 and 42 hours of incubation in either treatment. Control cultures did not receive any treatment. (A) Pictures of representative cells taken from each condition at each time point. Within this time frame an apparent difference in the rate of change of both the total dendritic length and number of branching points was observed (5B,D, respectively). (C,E) The differences in the actual rate expressed as percentage of increase in length and number of branching points, respectively, with respect to the measurements taken at 18 hours (lgt F(2,27)=15.86, P<0.0001; NB F(2,27)=11.51, P=0.0002).