Fig. 3. Pollen development is restored and anthers dehisce in knuckles plants grown at 16°C. (A) Morphological comparison of wild-type and knu anthers at the time of flower opening taken from plants grown at 25°C and 16°C. Lateral and adaxial views are shown. (B) Section through knu anther from an early stage 10 flower taken from a plant grown at 25°C, showing post-tetrad microspores and a semi-intact tetrad. At this stage knu anthers and developing pollen were indistinguishable from wild-type. (C) Cross-section through knu anther taken from the open flower of a plant grown at 25°C. Stamen is non-dehiscent and mature pollen grains are absent. Scale bars: 50 µm in B,C. dpg, degenerating pollen grains; lc, locule; ms, microspore; mit, microspore tetrad.