Fig. 6. Motif sequence comparisons between KNUCKLES and other Arabidopsis C2H2 zinc finger proteins. (A) Alignment of C2H2 zinc fingers and adjacent basic residues. The first line consensus shown in red was taken from Evans and Hollenberg (Evans and Hollenberg, 1988). Cysteine and histidine residues responsible for zinc binding are highlighted in yellow. The sub-motif QALGGH is present in a majority of plant zinc finger proteins (Takatsuji, 1999). At5g48890.1, a protein of unknown function, is 77% identical to KNUCKLES through the aligned region. AtZFP10 has been studied by Dinkins et al. (Dinkins et al., 2002), and is part of a family of approximately 30 small, single-fingered proteins identified by those authors and Tague and Goodman (Tague and Goodman, 1995). (B) Alignment of EAR and EAR-like motifs. ZAT11 contains a consensus EAR motif (L/FDLNL/FXP) as defined by Ohta et al. (Ohta et al., 2001), whereas the others are EAR-like as described by Hiratsu et al. (Hiratsu et al., 2002). Residues conserved in all five proteins shown in blue and indicated below alignment by (^*); 5/5 conservative by (5), and 4/5 conserved or conservative by (4), 3/5 conserved or conservative by (3). Numbers at right indicate position of motif relative to total protein length. Accession numbers as follows: KNUCKLES (NP_196905), SUPERMAN (S60325), At5g48890.1 (NM_124266), AtZFP10 (AAC23644), ZAT11 (F84792).