Fig. 9. Complementation and GUS reporter constructs and their effects in transgenic Arabidopsis. (A) Complementation constructs. Plasmids listed far left. Binary T-DNA parent plasmids shown in parentheses. Centre, inserts are depicted as coloured bars. KNU coding sequences are shown in red, and flanking genomic sequences are shown in yellow. Far right, hygromycin-resistant knu transgenics were scored as being fully or partially complemented or uncomplemented based on restoration of fertility and morphological criteria. (B) GUS reporter constructs. The uidA gene is shown as a blue bar. Far right, cartoons representing stage 6 and 9 flower buds, early ovules, and leaf and stem are shaded blue to approximate patterns of GUS staining at the stages depicted in multiple independent lines containing each construct. Scale in kilobases (Kb). H, HindIII; N, NcoI; P, PstI; X, XbaI; X^m, methylation-sensitive XbaI site.