Fig. 6. Expression of duf-lacZ in the adult thoracic myoblast pool. (A-C) Expression of duf-lacZ in the wing disc-associated myoblasts. (A) Faint duf-lacZ expression in the myoblasts that adhere to the notal region of the wing disc. The region outlined by the black box is magnified in B and C. (B,C) Twist-expressing myoblasts (red; B) that also express duf-lacZ (green; C). (D-F) Co-localization of duf-lacZ with 1151Gal4-expressing myoblasts. (D) Confocal image of a 12-hour APF hemithorax showing the 1151-GAL4 expressing myoblasts of the developing DVM II fibres. 1151Gal4-driven expression is revealed by UAS-nls-GFP, with duf-lacZ in the background. Each arrowhead indicates a nucleus, which is larger in size than the other nuclei within the syncitium. (E) The duf-lacZ-expressing myoblasts of DVM II, assayed by anti-ß-galactosidase labeling. These two nuclei are the same as those indicated by arrowheads (D). (F) Merged image showing the co-localization of duf-lacZ (red) with 1151GAL4 (green). (G-I) Co-localization of duf-lacZ with Vg. (G) Confocal image showing Vg-expressing myoblasts that have fused to, or have swarmed over, the DVM II syncitium. Vg expression is observed by anti-Vg labeling on a 12-hour APF duflacZ pupa. Amidst the cluster of myoblasts, arrowheads indicate the myoblasts that also express duf-lacZ. (H) Anti-ß-galactosidase labeling reveals the duf-lacZ-expressing myoblasts of DVM II in the same preparation. (I) Merged image showing the co-localization of Vg with duf-lacZ. Scale bar: 50 µm for B,C; 30 µm for D-I.