Fig. 2. Interactions between Ds and Ft. (A) S2 cells transformed with gfp (green) alone do not adhere. (B) Cells co-transfected with ft and gfp do not adhere. (C) Cells cotransfected with ds, ft and gfp form large clusters (arrowheads). (D) The addition of 1 mM EGTA to cells co-transfected with ds, ft and gfp blocks adhesion. (E,F) Cells transformed with only ds (E) or ft (F) have only low levels of Ds or Ft on the cell surface. (G-J) Mixtures of cells separately transformed with ft (I, anti-Ft, red) and with ds and gfp (H, anti-Ds, blue; J, GFP, green) adhere. Adhering cells have higher levels of anti-Ds and anti-Ft staining at the interface between ds-expressing and ft-expressing cells (G-I, arrows), but low levels of anti-Ds staining at the interface between ds-expressing cells (G,H; arrowheads). (K-N) Changes in Ft or Ds stability and distribution induced by misexpression of ds or ft in late third instar wing discs. (K,L) Misexpression of ds in the posterior using en-gal4 (K) or in clones using FLPout-gal4 (L; identified by UAS-GFP, green) causes heightened anti-Ft staining at the cell surface. Wild-type cells at the interface with the ds-expressing clone (L) had a lower than normal anti-Ft staining, but staining was higher at the interface with the clone. (M,N) Misexpression of ft in the posterior using en-gal4 (M), or in clones using FLPout-gal4 (N; identified by UAS-GFP, green), causes heightened anti-Ds staining at the cell surface. Wild-type cells at the interface with the ft-expressing clone (N) had a lower than normal anti-Ds staining, but staining was higher at the interface with the clone. This was noted especially in clones in the prospective notum and wing hinge. Some ft-misexpressing filopodia or cell remnants extend from the clone (arrowheads) and have high levels of Ds.