Fig. 4. Wing hair polarity in regions of uniform ds misexpression. (A-D) Diagrams of hair polarity in adult wings. (A) ds⁰⁵¹⁴². (B) engal4/+; UAS-ds/+. Hair polarity is normal except near the boundary of misexpression. (C) tub-gal4/UAS-ds. Hair polarity is normal. (D) ds⁰⁵¹⁴²; tub-gal/UAS-ds. Hair polarity is rescued (compare with A), except in the proximal wing. (E,F) Comparison of anti-Ds staining in the proximal region of 5 hour AP wings in wild type (E) and tub-gal4/UAS-ds (F). Wings were stained in the same well and photos were taken using identical settings. (G-J) Comparison of hair polarity between ds⁰⁵¹⁴² (G,I) and ds⁰⁵¹⁴²; tub-gal/UAS-ds (H,J) wings. Uniform ds expression rescues the PCP defect in the distal region between L3 and L4 (G,H), but not in the proximal region just posterior to L1 (I,J).