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Fig. 3. SOX9 expression in human embryo GI system. (A-E) Immunohistochemistry analyses using anti-SOX9 antibodies on paraffin sections from a 7.5-week-old (7.5 w) human embryo. (A) Lung. (B) Pancreas. (C) Posterior region of the stomach. (D) Small intestine. (E) Rectum. Expression of SOX9 protein is found in human viscera epithelial layers and there is exclusive mesodermal expression of SOX9 protein at the pyloric level in the stomach (red arrow, C). (F) Immunohistochemistry with anti-SOX9 antibodies on paraffin wax sections from prenatal human small intestine (22 w). Note the expression of SOX9 in the proliferative compartment of the villi (red arrow, F). mg, midgut; panc, pancreas; prenat, prenatal; pyl, pyloric structure; rect, rectum; si, small intestine; sto, stomach.