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Fig. 4. Expression of E subgroup Sox genes, Bmp4 and Nkx2.5 in 7-day-old (E7) chick stomach. (A) Whole-mount in situ hybridization of E7 dissected gut using antisense Sox9 riboprobe. Sox9 expression strongly demarcates the stomach from the duodenum and is only observed in the mesoderm of the pyloric sphincter (red arrow, A). (B,C) Whole-mount in situ hybridization of E7 dissected gut, using antisense Sox8 (B) and Sox10 (C) riboprobes. Sox8 and Sox10 expression in the stomach is restricted to ENS cells (B,C). (D,E) Whole-mount in situ hybridization of E7 dissected gut using antisense Bmp4 (D) and Nkx2.5 (E) riboprobes. Note that Bmp4 is expressed in the mesenchyme of the duodenum and the pylore (red arrow, D), but not in the gizzard. Nkx2.5 expression is observed only in the pyloric mesenchyme (red arrow, E). gizz, gizzard; pv, proventriculus; pyl, pyloric sphincter.