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Fig. 3. Sox2-expressing cells in the adult brain (DAB staining). (A,B) Origin of the rostral migratory stream in the top corner of the lateral ventricle. Both SOX2 immunostaining (brown) and Haematoxylin counterstaining (blue) of the same section identify a nuclear location. (C) SOX2 immunostaining of lateral ventricle ependyma. (D) SOX2 expression in choroid plexi. (E,F) SOX2-positive (brown) pyramidal cells in cortex (black arrowheads in E), with haematoxylin counterstaining (blue); F shows a higher magnification image. White arrowheads in E indicate SOX2-negative cells. (G) SOX2-positive cells in the striatum (brown). (H) Abundant SOX2-positive cells in the thalamus (brown) with light haematoxylin counterstaining (blue). (I) Occasional SOX2-positive cell (arrow) observed in the corpus callosum.