Fig. 9. Nestin- and GFAP-positive early precursors are severely reduced in mutant hippocampus. (A,C) Immunofluorescence detection of radially oriented nestin-expressing cells (bright green; white arrowheads) in wild-type (A) and mutant (C) hippocampus. (B,B',D,D') Immunohistochemical detection (DAB, brown) of GFAP-expressing cells in wild-type (B) and mutant (D) hippocampus. Note, in the enlargement of the boxed areas (B',D'), the severe reduction of GFAP-positive radially oriented cells within the dentate gyrus. (E,F) Double immunofluorescence detection of nestin (E,E',F,F'; green)/GFAP (E,E'',F,F''; red) double-positive precursor cells in the hippocampus dentate gyrus of wild-type (E) and mutant (F) mice. Double-positive cells are indicated by white arrowheads. The dotted lines define the dentate gyrus region. HIL, hilus.