Fig. 2. Notch signaling induces eyg transcription in eye disc. (A) eyg^M3-12/TM3 fly is an enhancer trap line with a P[lacW] inserted in the eyg locus. The mini-white reporter in P[lacW] reflected the eyg expression pattern and in eyg^M3-12/TM3 shows the eye pigmentation pattern in an anterior-central region of adult eye. (B) The reporter expression in eyg^M3-12/TM3 is reduced in the N^ts mutant background. The eggs were laid at 18°C, raised at 18°C for 5 days, then shifted to 29°C until adults eclosed. The eye size is reduced. (C-F) Clones of ectopic N^act (marked by GFP, green) caused tissue overgrowth in eye-antennal disc. (C) DIC image. (D,F) eyg-lacZ (eyg^M3-12, stained by anti-ß-galactosidase, red) expression is activated (arrow) within the N^act clones in eye discs except in the wg domain (arrowhead). (E) WG (stained by anti-WG, blue) is enhanced (arrowhead) within the wg endogenous expressing domain. (G-L) In most Su(H)^SF8 clones (marked by the absence of GFP, green) eyg-lacZ (eyg^M3-12, stained by anti-ß-galactosidase, red) expression were not detected. But in a few clones, the mutant cells at the border of the clone still expressed eyg-lacZ.