Fig. 3. eyg is induced at new DV border created by Ser- or Dl-expressing clones. (A-C) In Dl^rev10, Ser^RX82 and eyg^M3-12 triple heterozygous mutant, the eye size and eyg expression (eye pigmentation based on the mini-white reporter in eyg^M3-12) were both reduced (1% of flies are like A; 98% are like B; 1% are like C). (D-I) Ectopic expressing clones were marked by GFP (green). Discs were stained using anti-Elav (blue) and anti-ß-galactosidase (eyg^M3-12, red) antibodies. (D-F) When the Ser-expressing clone is located in the dorsal side near the DV midline of eye disc, eyg-lacZ is induced non-autonomously at the border of the clone (arrow). E,F are higher magnifications of D. (G-I) When the Dl-expressing clone is located in the ventral side near the DV midline of eye disc, eyg-lacZ is induced non-autonomously at the border of the clone (arrow). H,I are higher magnifications of G.