Fig. 8. Non-autonomous rescue of lamination at 50 hpf by wild-type cells transplanted into shh mutant embryos. Wild-type cells are unlabeled, but they carry the shh-GFP transgene (green). The inner plexiform layer is detected with phalloidin staining (red). (A) An example of a very small group of wild-type shh-expressing cells that lead to elevated levels of F-actin in adjacent cells (arrowhead) in the position where the IPL would normally form. (B) Higher magnification of the image in A. (C) Two groups of wild-type shh- expressing cells lead to local rescue of the IPL (arrowheads). (D) Higher magnification of the image in C. (E-G). Local rescue of the IPL by groups of wild-type cells expressing shh. In G, wild-type cells are labeled in blue, but rescue is only observed close to shh-GFP-expressing wild-type cells (arrowhead). (H) Large clones (blue) fail to rescue lamination if they do not contain shh-GFP expressing cells (green). (I) Wild-type cells expressing shh-GFP in the RPE fail to rescue lamination. (I') The GFP single channel of the image shown in I, showing shh-GFP-expressing cells in the RPE (arrowhead, inset).