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Fig. 5. Overexpression of Pea3 results in ectopic Scx expression in the dermomyotome and dorsal sclerotome. (A) Whole-mount in situ hybridization for Scx following infection with RCAS-Pea3. (B,C) Section in situ hybridization for Scx on frontal sections of control (B) or RCAS-Pea3-infected embryos (C). (D) Detection of viral infection using 3C2 antibody on section shown in C. (C) Blue arrow indicates ectopic Scx in dorsal sclerotome. (E,H) Section in situ hybridization for Scx (E,F) or Pea3 (G,H) on frontal sections of control (E,G) or RCAS-FGF8-infected embryos (F,H). (I-P) Whole-mount in situ hybridization for Scx (I-L), Myf5 (M,N) or Fgf8 (O,P) on trunks cultured in either DMSO (control) (I,K,M,O) or 30 µM SU5402 (J,L,N,P). Trunks shown in J and L were infected with RCAS-Pea3 on their right sides. Dm, dermomyotome; M, myotome; Sc, sclerotome.