Fig. 3. lg1 and lg2 expression in Wab1 leaves. RT-PCR gel blot analysis of lg1 and lg2 expression in 4-week-old seedlings of wild-type and Wab1/+ at the following stages of leaf development: SAM including P1-5 (S), P6-8 pre-ligule region (PL), P6-8 blade (B), P9-10 ligule region (L). Lanes 9-16 are as for lanes 1-8 using a four-fold dilution of cDNA. Control with no cDNA included in PCR (C). Control amplification of ubiquitin (ub) indicates equal amounts of cDNA present in each sample.