Fig. 1. Ectopic eyes in an ey² mutant background. For each panel (A-L), the UAS transgene that is driven by the dpp^blink-Gal4 transgene in an ey² mutant background is indicated in the lower right of the panel. (A-D) Phenotype of adult flies carrying the UAS transgene indicated. Misexpression of toy (A), toyHD (B), sey (C) and seyHD (D) leads to the induction of ectopic eyes in an ey² mutant background. (E-L) Early and late marker genes are induced during ectopic eye development in the absence of endogenous ey. (E-H) lacZ expression of third instar leg discs carrying the so-enhancer trap, in addition to the dpp^blink-Gal4 transgene and the UAS-construct indicated in an ey² mutant background. All four transgenes were able to induce lacZ expression. (I-L) Rhodopsin 1 expression in ectopic eyes. Rhodopsin 1 was monitored with an -Rhodopsin 1 antibody on cryosections of ectopic eyes induced by the UAS transgene indicated driven by dpp^blink-Gal4 in an ey² mutant background. Rhodopsin 1 was found to be expressed in ectopic eyes induced by all four transgenes. Arrows indicate the retina.