Fig. 4. vent and vox are direct transcriptional targets of Wnt8/ß-catenin signaling. (A) vent and vox expression in control (a,c) or treated (b,d) embryos. Arrows in panels b and d indicate ectopic expression upon induction of GR-LEFN-ßCTA with DEX. (B) Percentage of embryos displaying ectopic vent or vox domains (y-axis) upon treatment with CHX alone, DEX alone, or CHX+DEX (x-axis). The control bar represents embryos injected with GR-LEFN-ßCTA and treated with ethanol (n=109 for vent, n=177 for vox). Error bars represent s.e.m. When performing the ² test on DEX versus DEX+CHX means, P>0.05 for both vent and vox, meaning that the difference between the means is not statistically significant.